FIELD PHYTOPROTECTION OF Coffea arabica MOTHER PLANTS, DISINFECTION AND CALLOGENESIS INDUCTION

In Chiapas, Mexico, there are high-yielding coffee plants adapted to the local climatic conditions. The presence of microorganisms in explants makes developing protocols for cloning these genotypes vitro difficult. objective this work was evaluate how field and microorganism management affected asepsis induction callogenesis leaf agronomically important plants. Drought-tolerant were obtained from aseptic cell cultures using a participatory study, agronomic, techniques. Five accessions (ITTGj 1-5) selected 21-year-old Borbon cultivar. present study conducted over two flowering cycles, evaluating effect on explant first callogenic response caused by culture medium, transport solution, genotype, second. Sixty days before collection, biweekly applications calcium polysulfide (10 % v v-1), copper oxychloride (2 g L-1), Zingiber officinale extract (20 L-1) reduced fungal contamination 100 bacterial 90 %. Field transportation 200 mg L-1 citric acid ascorbic solution oxidation cultures. Proembryogenic friable corns formed with combination 4.4 µM BAP (6-benzylaminopurine) 7.25 2,4-D (2,4-dichlorophenoxyacetic acid) at 45 culture, genotype four showing best response. effective establishment Coffea arabica vitro, used an appropriate growth regulator callogenesis.